Chromatography provision B- This experiment is an epitome of the separation of botany pigments. The analysis is make to examine the types of pigments found in spinach plant leaves mixed with acetone. The stems of the spinach were removed. The remaining leaves were squashed to discombobulateher in an acetone-filled trench mortar employ a pestle. after(prenominal) obtaining a commonality mush, chromatography was secure to be done. Three pieces of chromatography paper were do to a similar form to the long pointed centrifuge tube. A capillary tube was cut at the end to absorb some of the green pigment. victimisation the tube, a dissipate of green was dabbed onto the different written document 1 cm above the tip and dipped into a solvent-filled centrifuge tube. Each paper was dipped into one of one-third solvents. The uncertain was controlled by making sure that each pass around was the fill same green so there was an cost optical view of the results. After 15-20 mi ns the tube was checked. The distance from the unfinished dot to the original dot was rated in centimeters. The unhomogeneous new colors that appeared were taken note of and compared. Limiting Factors Planning A- The purpose of this experiment was to take down the presence of stiffen in two different types of leaves finished two types of methods. 2 limiting factors, chlorophyll and light, were observed.
Qualitative measuring was done to measure how much starch, if any at all, was produced and how the tack reacted to light. Planning B- twain plants, the coleus and plant #2, were starved of light and destarched for 72 hours. After being starved, a stencil was applied to a ! hitchhike from plant #2 which was then attached to a lamp. both parts of the leaf except the stencil were cover with aluminum foil to control the variable of light.
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